Određivanje antioksidacijskog djelovanja odabranih vrsta roda Salvia L.

master's thesis

• Title (english): Determination of antioxidant activities of selected Salvia species
• Author: Doris Škugor
• Mentor: Sanda Vladimir-Knežević (mentor)
• Committee member: Sanda Vladimir-Knežević (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Renata Jurišić Grubešić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2020-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovog diplomskog rada istraženi su prisutnost i antioksidacijski učinak fenolnih spojeva u
metanolnim i etanolnim ekstraktima listova odabranih vrsta roda Salvia s područja Hrvatske (S.
brachyodon, S. fruticosa, S.glutinosa, S. nemorosa, S. officinalis, S. pratensis, S. sclarea i S.
verticillata). Primjenom metode tankoslojne kromatografije dokazana je prisutnost ružmarinske
kiseline i luteolin-7-O-glukozida u listovima svih uzoraka. Kvercitrin je prisutan samo u vrsti S.
verticillata, a rutin u vrstama S. brachyodon, S. fruticosa i S. officinalis. Antioksidacijski učinak
etanolnih ekstrakata ispitan je pomoću tri spektrofotometrijske metode u usporedbi s troloksom i
EDTA. Utvrđena je sposobnost hvatanja DPPH radikala (IC50: 3,88-7,93 μg/mL), redukcije iona
željeza(III) ( IC50: 9,43- 18,36 μg/mL) te keliranja iona željeza(II) ( IC50: 163,55-1592,53μg/mL).
Dobiveni rezultati pokazuju da istraživane biljne vrste sadrže flavonoide i fenolne kiseline koji
doprinose njihovim antioksidacijskim učincima.

Abstract (english)

The presence of phenolic compounds and their antioxidative effect in methanolic and ethanolic leaf
extracts of selected species of the Salvia genus from Croatia (S. brachyodon, S. fruticosa,
S.glutinosa, S. nemorosa, S. officinalis, S. pratensis, S. sclarea and S. verticillata) were studied in
this diploma thesis. The presence of rosmarinic acid and luteolin-7-O-glucoside was detected by
thin-layer chromatography in all leaf samples. Quercitrin was present only in the S. verticillata
extract and rutin was detected in leaf extracts of S. brachyodon, S. fruticosa and S. officinalis. The
antioxidative effects of ethanolic extracts were determined by using three spectrophotometric
methods in comparison to trolox and EDTA. The abilities of scavenging DPPH radical (IC50: 3.88-
7.93 μg/mL), reduce ferric ions (IC50: 9.43-18.36 μg/mL) and chelate ferrous ions were determined.
The obtained results have shown that the studied plant species contain flavonoids and phenolic acids
which contribute to their antioxidative effects.

• Keywords: Salvia; flavonoidi; fenolne kiseline; antioksidacijsko djelovanje
• Keywords (english): Salvia; flavonoids; phenolic acids; antioxidant effects
• Language: croatian
• URN:NBN: urn:nbn:hr:163:121402
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-09-29
• Created on: 2022-08-26 13:18:58