| Abstract | Cilj istraživanja
Cilj ovog specijalističkog rada je napraviti detaljan pregled i sistematizaciju znanstvenih
studija u kojima je ispitan utjecaj betulina iz kore bijele breze na zacjeljivanje rana te obraditi
rezultate provedenih istraţivanja kako bi se stekao uvid u recentna znanstvena saznanja
vezana za njegovu učinkovitost i sigurnost primjene.
Materijali i metode
Pretraţene su elektroničke baza podataka PubMed, Scopus, Web of Science. Pretragom su
obuhvaćene znanstvene studije koje su ispitivale utjecaj betulina na zacjeljivanje rana. U
pretraţivanju su korištene ključne riječi: rana (engl. wound), opeklina (engl. burn), betulin,
episalvan te oleogel-S10. Glavni kriterij odabira radova uključenih u ovaj rad bilo je
ispitivanje učinka na zacjeljivanje rana, opekline ili transplatirano tkivo. Pretragom baze
PubMed dobivena su 74 rada od kojih su 22 odgovarala odabranim kriterijima, dok se
pretragom baze Scopus i Web of Science dobilo 84 odnosno 46 radova od kojih je 26 odnosno
24 odgovaralo odabranim kriterijima. Nakon uklanjanja duplikata preostalo je 28 istraţivanja
koja su uključena u ovaj specijalistički rad. Za odabrane znanstvene radove bili su dostupni
cjeloviti tekstovi ili iznimno saţeci na engleskom jeziku. Radovi su obraĎeni neovisno o
datumu objave.
Rezultati
Betulin, u formulaciji oleogela, je ubrzao zacjeljivanje rana uzrokovanih opeklinama drugog
stupnja i transplantacijom tkiva, a njegova primjena rezultirala je manje vidljivim oţiljcima.
Veliki potencijal pokazao je i u zacjeljivanju rana kod pacijenata oboljelih od Epidermolysis
bullose te ranama uzrokovanim CO₂ laserom. Osim u formulaciji oleogela, betulin je pokazao
jednaku učinkovitost i u formulaciji V/U pjene, koja bi u praksi mogla imati prednost zbog
jednostavnije primjene i eventualne bolje suradljivosti pacijenata posebno kod kroničnih rana.
Na molekularnoj razini betulin djeluje na upalnu fazu zacjeljivanja rana regulacijom upalnih
citokina, kemokina i ciklooksigenaze, a utječe i na migraciju keratinocita. Ispitivanjem na
stanicama keratinocita i fibroblasta dijabetičara betulin je pokazao potencijal. Slično tomu,
oblozi za rane s nanovlaknima polivinil alkohola i triterpenskim ekstraktom i s nanočesticama
betulina i cinkova oksida pokazali su pozitivan učinak na zacjeljivanje opeklina.
Zaključak
Rezultati znanstvenih istraţivanja ukazuju na to da betulin iz kore bijele breze ubrzava
zacjeljivanje rana i smanjuje vidljivost oţiljaka. Navedeni rezultati potvrĎeni su za nekoliko
farmaceutskih oblika |
| Abstract (english) | Objectives
The aims of this final paper are to make a detailed review and outline of research studies
examining the impact of betulin from the birch bark on wound healing and conduct an
analysis of the research results so as to gain insight into the recent scientific knowledge
related to effectiveness and application safety of betulin.
Materials and methods
The electronic databases PubMed, Scopus, and Web of Science were searched. The research
included scientific studies that have been dealing with the impact of betulin on wound
healing. The keywords used in the research were: wound, burn, betulin, episalvan, and
oleogel-S10. The main criterion for selecting the articles included in this study was the study
of the effect on wound healing, burns, or graft tissue. The PubMed Base search resulted in 74
papers out of which 22 met the selected criteria, while the Scopus and Web of Science
database search resulted in 84 and 46 papers, out of which 26 and 24 met the selected criteria,
respectively. After the removal of the duplicates, there remained 28 studies that were a part of
this final paper. For a selection of articles, there were complete texts or summaries in English
available. The papers were processed for the research regardless of the date of publication.
Results
Betulin, in the formulation of oleogel, accelerated the healing of wounds caused by seconddegree burns and tissue transplantation, and its application resulted in less visible scars. It has
also shown great potential in wound healing in patients with Epidermolysis bullosa and
wounds caused by CO₂ laser. In addition to the formulation of oleogel, betulin has shown the
same efficacy in the formulation of V/U foam, which could in practice be advantageous due
to simpler application and possibly better patient compliance, especially in chronic wounds.
At the molecular level, betulin acts on the inflammation of wound healing by regulating
inflammatory cytokines, chemokines, and cyclooxygenase, and affects the migration of
keratinocytes, as well. In a study in keratinocyte cells and diabetic fibroblasts, betulin showed
healing potential. Similarly, wound dressings with polyvinyl alcohol and triterpene extract
nanoparticles and betulin and zinc oxide nanoparticles showed a positive effect on burn
healing.
Conclusion
The results of scientific research indicate that betulin from the birch bark accelerates wound
healing and reduces the visibility of scars. The above results were confirmed for several
pharmaceutical forms. |
