Simultano određivanje željeza i bakra u serumu djece FAAS-om

Scientific paper - Preliminary note / Short communication

• Title (english): Simultaneous determination of iron and copper in children's sera by FAAS
• Author: Svjetlana Luterotti
• Author: Tončica Vukman Kordić
• Author: Slavica Dodig
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of analytical chemistry)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (croatian)

Predložena je nova jednostavna metoda plamene atomsko-apsorpcijske spektrometrije (FAAS), za simultano određivanje željeza i bakra u serumu djece. Ona se temelji na predobradbi uzorka u jednom koraku (deproteinizacija s 3 mol L–3 HCl u odnosu 1:1) i kalibraciji u jednom koraku sa standardom pripravljenim u 1.5 mol L–3 HCl.
Tijekom optimizacije metode primijenjen je multifaktorski dizajnirani eksperiment. Preporučena metoda osigurava ispravnost, osjetljivost i preciznost usporedljivu onima referentnih metoda. Novi je pristup jednostavan i brz; on štedi i vrijeme i reagense i uzorke, pri čemu je potonje posebno važno u dječjoj dijagnostici.

Abstract (english)

A new and simple flame atomic-absorption spectrometric (FAAS) method is proposed for simultaneous determination of iron and copper in children's sera. It is based on single-step sample pretreatment (deproteinization with 3 mol L–1 HCl, ratio 1:1) and single-step calibration using 1.5 mol L–1 HCl standard. During method’s optimization a short multifactorial design experiment was used. The proposed method assures accuracy, sensitivity and precision comparable to that of the reference methods. The new approach is simple and time-, labour- and serum-saving, the latter being especially important in pediatric diagnostics.

• Keywords (croatian): željezo; bakar; dječji serum; FAAS; određivanje u jednom koraku
• Keywords (english): iron; copper; children´s serum; single-step determination; FAAS
• Language: english
• Publication type: Scientific paper - Preliminary note / Short communication
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Acta Pharmaceutica
• Numbering: vol. 61, no. 1, pp. 93-102
• p-ISSN: 1330-0075
• e-ISSN: 1846-9558
• DOI: https://doi.org/10.2478/v10007-011-0008-4
• URN:NBN: urn:nbn:hr:163:351535
• Publication: 2011-03-01
• Document URL: http://hrcak.srce.hr/63379
• Type of resource: Text
• Access conditions: Open access
• Created on: 2017-06-14 15:19:27