Razvoj i validacija nove sHSS-GC-FID metode za određivanje sadržaja lako hlapljivih sastavnica u dodacima prehrani kod upalnih bolesti crijeva

master's thesis

• Title (english): Development and validation of new sHSS-GC-FID method fo determination of volatile compounds in dietary supplements used in inflammatory bowel disease
• Author: David Skendrović
• Mentor: Ana Mornar Turk (mentor)
• Committee member: Ana Mornar Turk (predsjednik povjerenstva)
• Committee member: Biljana Nigović (član povjerenstva)
• Committee member: Toma Keser (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2019-05-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Upalne bolesti crijeva, Crohnova bolest i ulcerozni kolitis, su autoimune, idiopatske bolesti
karakterizirane kroničnom upalom lociranom u gastrointestinalnom sustavu. Uz klasičnu
farmakoterapiju koju čine aminosalicilati, kortikosteroidi i biološka terapija, u novije doba pacijenti
sve više pribjegavaju različitim dodacima prehrani kao adjuvantnoj terapiji. Kurkuma je svojim
protuupalnim i imunosupresivnim svojstvima uvjerljivo etablirala svoje mjesto u plejadi
tradicionalne farmakoterapije i pokazala obećavajuće rezultate u liječenju ove skupine bolesti. No u
zadnjih dvadesetak godina tržište je preplavljeno različitim formulacijama kako kurkume, tako i
ostalih dodataka prehrani što predstavlja problem zbog puno slabije zakonske regulative u razvoju i
kontroli kakvoće ovih proizvoda. U svjetlu navedenih činjenica jasna je potreba za razvojem
jedinstvene analitičke metode za simultano određivanje glavnih sastojaka i onečišćenja prisutnih u
formulacijama dodataka prehrani. U ovom radu prikazan je razvoj i validacija sHSS-GC-FID metode
za simultano određivanje etanola, metanola, acetona, izopropanola, n-propanola, t-butanola,
izobutanola i n-butanola te njezine primjene u analizi tinkture kurkume. Uz primjenu unutarnjeg
standarda dobiveni su pouzdani rezultati te je kvantifikacija uspješno izvršena i ispitivani uzorak je
zadovoljio sve kriterije propisane ICH smjernicama što ga čini sigurnim za upotrebu u terapijske
svrhe.

Abstract (english)

Inflammatory bowel diseases, Crohon's disease and ulcerative colitis, are autoimmune, idiopathic
diseases characterized by chronic inflammation in the gastrointestinal tract. Beside classical
pharmacotherapy which consists of aminosalicylates, corticosteroids, immunosuppressants and
biological therapy, in the modern era patients are keen to usage of different dietary supplements as
adjuvant therapy. Curcuma longa with its anti-inflammatory and immunosuppressive properties has
assured its position in the pleads of traditional pharmacotherapy and has promising properties in the
future treatment of this type of diseases. In the last twenty years, the market has been flooded with
variety of formulations of dietary supplements, which is considered a significant problem due to
weaker legislation in their development and quality control. In the light of these facts, the need for
development of unique analytical method for simultaneous determination of main ingredients and
its impurities in the dietary supplements is needed. The aim of this research is to develop and validate
a sHSS-GC-FID method for simultaneous determination of ethanol, methanol, acetone, isopropanol,
n-propanol, t-butanol, isobutanol and n-butanol and to apply it in analysis of turmeric tincture. With
the usage of internal standard, the applied method has proven its analytical value and quantification
has been successfully achieved. The tested sample has satisfied all the criteria proposed by ICH
guidelines and has proven to be safe for therapeutical purposes.

• Keywords: upalne bolesti crijeva; dodaci prehrani; sHSS-GC-FID; hlapljive sastavnice
• Keywords (english): inflammatory bowel disease; dietary sumplements; sHSS-GC-FID; volatile compounds
• Language: croatian
• URN:NBN: urn:nbn:hr:163:205920
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-28 12:21:07