Capillary electrophoresis determination of pravastatin and separation of its degradation products

Scientific paper - Original scientific paper

• Author: Biljana Nigović
• Author: Ivana Vegar
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (english)

A capillary zone electrophoresis method for pravastatin determination was developed and validated.
Rapid migration of negatively charged pravastatin molecule was obtained in alkaline
buffer by the application of electric field of 30 kV. Influence of the pH value and ionic strength
of running buffer, applied voltage and capillary temperature on mobility and sensitivity was
evaluated. Detection wavelength was set to 237 nm. The method was applied to the determination
of the drug in pharmaceutical dosage form. Pravastatin is a δ-hydroxy acid, which is prone
to lactonize and epimerize in a pH-dependent manner. Micellar electrokinetic chromatographic
approach was chosen to develop a method able to separate pravastatin and its degradation
products in acidic media. The proposed method allows baseline separation of hydroxy acid and
neutral lactone forms of the drug that appear as interconversion products depending on the pH
value.

• Keywords (english): pravastatin; capillary zone electrophoresis; pharmaceuticals; micellar electrokinetic chromatography; degradation products
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Croatica Chemica Acta
• Numbering: vol. 81, no. 4, pp. 615-622
• p-ISSN: 0011-1643
• e-ISSN: 1334-417X
• URN:NBN: urn:nbn:hr:163:647858
• Publication: 2008
• Document URL: https://hrcak.srce.hr/31187
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-04-21 15:55:19