In vitro ispitivanje oslobađanja azitromicina iz liposoma uklopljenih u kitozanski hidrogel

master's thesis

• Title (english): In vitro release study of azithromycin from deformable liposomes incorporated into chitosan gel
• Author: Marina Jug
• Mentor: Željka Vanić (mentor)
• Committee member: Željka Vanić (predsjednik povjerenstva)
• Committee member: Anita Hafner (član povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2019-02-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Cilj ovog rada bio je provesti in vitro ispitivanje oslobađanja azitromicina iz konvencionalnih i deformabilnih liposoma uklopljenih u kitozanski gel, inovativne formulacije namijenjene lokalnoj primjeni na kožu. Liposomi su pripremljeni metodom hidratacije suhog fosfolipidnog sloja uz ekstruziju kroz polikarbonatne membrane od 400 i 200 nm, dok je gel izrađen iz kitozana velike molekulske mase. Deformabilni liposomi bili su manjeg srednjeg promjera, ali nešto većeg indeksa polidisperznosti od konvencionalnih liposoma, što je posljedica veće elastičnosti fosfolipidnih dvoslojeva. Oba tipa liposoma imali su negativni zeta potencijal kao preduvjet fizičke stabilnosti liposomskih disperzij. Liposomi, odijeljeni od neuklopljene frakcije azitromicina, umiješani su u 1.5% kitozanski gel u koncentraciji 30% (w/w). In vitro ispitivanja oslobađanja azitromicina iz liposoma uklopljenih u kitozanski gel potvrdila su produljeno oslobađanje antibiotika u usporedbi s kontrolnim gelom (otopina azitromicina u gelu). Usporedba profila oslobađanja antibiotika iz različitih tipova liposoma-u-gelu pokazala je sporije oslobađanje iz konvencionalnih liposoma zbog manje propusnosti membrane za ukloljeni azitromicin u odnosu na deformabilne liposome.

Abstract (english)

The aim of this study was to evaluate in vitro release of azithromycin from conventional and deformable liposomes incorporated into chitosan gel. Liposomes were prepared by film hydration method and extrusion through polycarbonate membranes of 400 and 200 nm, while the gel was prepared using high molecular weight chitosan. Deformable liposomes were of smaller size, but somewhat higher polydispersity index than conventional liposomes. Both types of liposomes had negative zeta potentials as a precondition for physical stability of liposomal dispersions. Liposomes, separated from unentrapped azithromycin, were mixed into 1.5% chitosan gel at a concentration of 30% (w/w, liposomes/gel). In vitro assessment of azithromycin release from the different liposomes incorporated into chitosan gel have confirmed prolonged release of the drug from the both types of liposomes compared to control gel (azithromycin solution incorporated in gel). Comparison of the release profiles of azithromycin from the different types of liposomal gels showed slower release from conventional liposomes.

• Keywords: azitromicin; deformabilni liposomi; in vitro oslobađanje; dermalna primjena; kitozan
• Keywords (english): azithromycin; deformable liposomes; in vitro release; chitosan
• Language: croatian
• URN:NBN: urn:nbn:hr:163:588396
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-04-21 11:20:06