Određivanje količine flavonoida i fenolnih kiselina te antiagregacijskog učinka u vrstama Calamintha nepeta (L.) Savi, Lavandula angustifolia Mill. i Ocimum basilicum L.

master's thesis

• Title (english): Quantitative analysis of flavonoids and phenolic acids and determination of anti-aggregation effect in species Calamintha nepeta (L.) Savi, Lavandula angustifolia Mill. and Ocimum basilicum L.
• Author: Marko Parlov
• Mentor: Željan Maleš (mentor)
• Committee member: Željan Maleš (predsjednik povjerenstva)
• Committee member: Mirza Bojić (član povjerenstva)
• Committee member: Biljana Nigović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical botany); Zagreb
• Defense date and country: 2018-05-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Ovo istraživanje provedeno je na odabranim biljnim vrstama porodice Lamiaceae: Calamintha nepeta (L.) Savi, Lavandula angustifolia Mill. te Ocimum basilicum L.
Biljke su skupljene u Farmaceutskom botaničkom vrtu ˝Fran Kušan˝ Farmaceutsko-biokemijskog fakulteta Sveučilišta u Zagrebu te u Orebiću na poluotoku Pelješcu.
Sadržaj flavonoida i fenolnih kiselina određen je Uv-Vis spektrofotometrijskom metodom, dok je antiagregacijski učinak određen metodom impedancijske agregometrije.
Dobiveni rezultati pokazuju varijacije i u sastavu fenolnih spojeva i u antiagregacijskom učinku ovisno o biljnoj vrsti i geografskom nalazištu na kojem su biljke prikupljene.
Udio flavonoida u biljkama kretao se od 0,12% do 0,30%. Najveći udio flavonoida (0,30%) je zapažen kod vrste L. angustifolia ubrane u Orebiću na mjestu Škvar.
Udio fenolnih kiselina u biljkama kretao se od 4,78% do 10,82%. Najveći udio fenolnih kiselina također je zapažen kod vrste L. angustifolia ubrane u Orebiću na mjestu Škvar. Najpotentniji antiagregacijski učinak definiran statistički značajnom antiagregacijskom koncentracijom, pokazao je uzorak flavonoidnog ekstrakta bosiljka (O. basilicum) s pripadajućom vrijednosti statistički značajne antiagregacijske koncentracije od 0,02 μmol/L.
Budući da ne postoji direktna korelacija udjela fenolnih spojeva u biljkama s antiagregacijskim učinkom istih, pretpostavlja se da antiagregacijskom učinku prvenstveno pridonosi vrsta fenolnog spoja sadržanog u biljkama, a zatim njegov udio.

Abstract (english)

This research was conducted to assess the effect of chosen plant species of Lamiaceae family: Calamintha nepeta (L.) Savi, Lavandula angustifolia Mill. and Ocimum basilicum L. on platelet aggregation.
Plants were collected in ˝Fran Kušan˝ Pharmaceutical botanical garden of the Faculty of Pharmacy and Biochemistry of Zagreb University, and in Orebić on the Pelješac peninsula.
Quantities of flavonoids and phenolic acids were determined by UV-Vis spectrophotometric method, and anti-aggregation effect was determined by using impedance aggregometry.
Results obtained in this research show variations in both, amount of phenolic compounds and anti-aggregation effect, depended on plant species and geographic location where they were collected.
Amount of flavonoids in plants was between 0.12% and 0.30 %. The highest contetnt of flavonoids (0.30%) was measured in L. angustifolia species, collected in Orebić, Škvar. Quantity of phenolic acids in plants was between 4.78% and 10.8%. The highest contetnt of phenolic acids was also measured in L. angustifolia species, collected in Orebić, Škvar. The most potent anti-aggregation effect defined by statistically significant anti-aggregation concentration was determined in flavonoid basil extract (O. basilicum) with corresponding statistically significant anti-aggregation concentration value of 0.02 μmol/L.
As small number of plant species has been analysed, direct correlation between amount of phenolic compounds in plant species and anti-aggregation effect can’t be defined, but it can be presumed that class of phenolic compound contributes to anti-aggregation more than the amount due to difference in potency of flavonoids and phenolic acids.

• Keywords: flavonoidi; fenolne kiseline; spektrofotometrija; antiagregacija; O. basilicum; L. angustifolia; C. nepeta; gorska metvica; lavanda; bosiljak
• Keywords (english): flavonoids; phenolic acids; spectrophotometry; anti-aggregation effect; O. basilicum; L. angustifolia; C. nepeta; mint; lavander; basil
• Language: croatian
• URN:NBN: urn:nbn:hr:163:918464
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-04-22 10:29:40