Primjena pektina iz komine mandarine u zelenoj sinetezi nanoselena

master's thesis

• Title (english): Application of mandarin pectin in green synthesis of selenium nanoparticles
• Author: Nela Predović
• Mentor: Dubravka Vitali Čepo (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Ivan Pepić (član povjerenstva)
• Committee member: Lovorka Vujić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Defense date and country: 2021-07-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Komina mandarine je otpad prehrambene industrije koji se u poslijednje vrijeme sve više prepoznaje kao
izvor različitih biološki vrijednih molekula. Jedan od najzastupljenijih spojeva s biološkom vrijednošću u
komini mandarine jest i pektin, koji općenito ima široku primjenu u prehrambenoj i farmaceutskoj
industriji. Cilj ovog rada je bio istražiti mogućnosti primjene sirove i pročišćene pektinske frakcije komine
mandarine u zelenoj sintezi nanoselena. Obje frakcije korištene su u 4 različite koncentracije, a dobiveni
rezultati uspoređeni su s rezultatima dobivenim primjenom komercijalno dostupnog pektina jabuke te
polivinilpirolidona kao često korištenog sintetskog sredstva za oblaganje. Fizikalno-kemijska
karakterizacija dobivenih nanočestica uključila je mjerenje zeta-potencijala te raspodjelu veličine čestica
primjenom dinamičkog raspršenja svjetlosti. Istražen je i utjecaj vrste/koncentracije sredstva za oblaganje
na ukupan prinos zelene sinteze te reduktivnu i antiradikalnu aktivnost dobivenih nanočestica selena.
Rezultati su pokazali da se i sirova i pročišćena pektinska frkacija komine mandarine može smatrati
pogodnim sredstvom za oblaganje u zelenoj sintezi nanočestica. Ovisno o promatranom parametru,
nanočestice selena dobivene inkapsulacijom pektinskom frakcijom komine mandarine usporedive su ili
bolje od nanoselena dobivenog inkapsulacijom PVP-om ili komercijalno dostupnim pektinom. Potrebna su
daljnja istraživanja kojima bi se dodatno usavršio protokol sinteze nanočestica selena uz pektine komine
mandarine kao sredstva za oblaganje kako bi se dobile čestice višeg antioksidacijskog potencijala te
promjera oko 100 nm.

Abstract (english)

Mandarin pomace is a waste of the food industry that has recently been recognized as a source of various
biologically valuable molecules. One of the most common compounds with biological value in mandarin
pomace is pectin, which is generally widely used in the food and pharmaceutical industries. The aim of this
study was to investigate the possibilities of applying the raw and purified pectin fraction of mandarin
pomace in green synthesis of selenium nanoparticles. Both fractions were used in 4 different concentrations
and the obtained results were compared with the results obtained using commercially available apple pectin
and polyvinylpyrrolidone as a frequently used synthetic coating agent. Physico-chemical characterization of
the obtained nanoparticles included measurement of zeta potential and particle size distribution using
dynamic light scattering. The influence of the type/concentration of the coating agent on the total yield of
green synthesis and the reductive and antiradical activity of the obtained selenium nanoparticles was also
investigated. The results showed that both raw and purified pectin fraction of mandarin pomace can be
considered as a suitable coating agent in green nanoparticle synthesis. Depending on the observed
parameter, selenium nanoparticles obtained by encapsulation with the pectin fraction of mandarin pomace
are comparable or better than selenium nanoparticles obtained by encapsulation with PVP or commercially
available pectin. Further research is needed to further refine the protocol for the synthesis of selenium
nanoparticles with mandarin pomace pectins as a coating agent to obtain particles with a higher antioxidant
potential and a diameter of about 100 nm.

• Keywords: mandarina; pektin; selen; nanočestice; zelena sinteza
• Keywords (english): mandarin; pectin; selenium; nanoparticles; green synthesis
• Language: croatian
• URN:NBN: urn:nbn:hr:163:386865
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-02-03 11:56:06