Fitokemijski sastav i antioksidacijski potencijal ploda američke borovnice (Vaccinium corymbosum L.)

master's thesis

• Title (english): Phytochemical composition and antioxidant potential of the american blueberry fruit (Vaccinium corymbosum L.)
• Author: Domagoj Mihoković
• Mentor: Biljana Blažeković (mentor)
• Committee member: Biljana Blažeković (predsjednik povjerenstva)
• Committee member: Hrvoje Rimac (član povjerenstva)
• Committee member: Marijan Marijan (član povjerenstva); MBZ: 335524
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2022-07-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovog diplomskog rada provedena su istraživanja fitokemijskog sastava i antioksidacijske učinkovitosti plodova američke borovnice (Vaccinium corymbosum L.) Prisutnost fenolnih kiselina, flavonoida i antocijana u plodu je dokazana metodom tankoslojne kromatografije. Spektrofotometrijskim određivanjima ustanovljeno je da etanolno-vodeni ekstrakt ploda sadrži 0,72±0,10 %, fenolnih kiselina, 0,43±0,02 %, antocijana, 0,66±0,03 % trjeslovina i 0,11±0,01 % flavonoida. S obzirom da su ove polifenolne sastavnice poznate kao prirodni antioksidansi u radu je ispitan antioksidativni potencijal ploda. Utvrđeno je da ekstrakt ploda američke borovnice ima izraženu sposobnost hvatanja DPPH radikala i pokazuje 50 %-tnu učinkovitost pri koncentraciji 97,41 μg/mL, dok je djelovanje na NO radikale slabo izraženo. Dobiveni rezultati doprinose dosadašnjim znanstvenim spoznajama o bioaktivnim sastavnicama i antioksidativnoj aktivnosti ploda američke borovnice uzgojene u Hrvatskoj.

Abstract (english)

Within the framework of this thesis, it was carried out the study of phytochemical composition and antioxidant activity of the fruits of the american blueberry (Vaccinium corymbosum L.). The presence of phenolic acids, flavonoids and anthocyanins in the fruit was confirmed by the thin-layer chromatography. Spectrophotometric determinations showed that the ethanol-water extract of the fruit contains 0.72±0.10% phenolic acids, 0.43±0.02% anthocyanins, 0.66±0.03% tannins and 0.11±0. 01% flavonoids, respectively. Given that polyphenol compounds are known as natural antioxidants, the antioxidant potential of the fruit was also evaluated. It was found that the american blueberry fruit extract has a pronounced ability to scavange DPPH radicals and shows 50% efficiency at a concentration of 97.41 μg/mL, while the effect on NO radicals is weak. The obtained results contribute to the current scientific knowledge on the bioactive compounds and antioxidant activity of the american blueberry fruit grown in Croatia.

• Keywords: Vaccinium corymbosum; plod američke borovnice; antioksidativni potencijal; antocijani; polifenoli
• Keywords (english): Vaccinium corymbosum; american blueberry fruit; antioxidant potential; anthocyanins; polyphenols
• Language: croatian
• URN:NBN: urn:nbn:hr:163:151249
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-07-21
• Created on: 2022-07-27 14:34:15