Ukupni polifenoli i antioksidativna aktivnost dodataka prehrani za liječenje upalnih bolesti crijeva

master's thesis

• Title (english): Total polyphenols and antioxidant activity of dietary supplements for the treatment of inflammatory bowel diseases
• Author: Ivona Pilić
• Mentor: Daniela Amidžić Klarić (mentor)
• Committee member: Daniela Amidžić Klarić (predsjednik povjerenstva)
• Committee member: Ana Mornar Turk (član povjerenstva)
• Committee member: Laura Nižić Nodilo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2022-09-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Upalne bolesti crijeva, Chronova bolest i ulcerozni kolitis, su kronične relapsirajuće bolesti koje pogađaju milijune ljudi diljem svijeta te narušavaju njihov svakodnevni život. Budući da polifenoli, sekundarni metaboliti biljaka, imaju antioksidativni i protuupalni učinak, cilj ovog rada bio je procijeniti kvalitetu dodataka prehrani za liječenje upalnih bolesti crijeva obzirom na sadržaj polifenola te odrediti antioksidativnu aktivnost istih. Sadržaj ukupnih polifenola u analiziranim uzorcima određen je spektrofotometrijskom metodom korištenjem Folin-Ciocalteu reagensa, dok je antioksidativna aktivnost procijenjena DPPH testom. Sadržaj polifenola značajno je bio veći u jednom multipreparatu tri biljne vrste: kurkuma, indijski tamjnanovac i crni papar. Nadalje, sadržaj ovih biološki aktivnih tvari u monopreparatima kurkume bio veći nego kod ostalih analiziranih monopreparata. Dobiveni rezultati upućuju na iznimnu kvalitetu dodataka prehrani za liječenje upalnih bolesti crijeva te se analizirani uzorci mogu smatrati dobrim izvorom polifenolnih sastavnica, dok je antioksidativna aktivnost ispitivanih dodataka prehrani u skladu sa sadržajem ovih biološki aktivnih sastavnica.

Abstract (english)

Inflammatory bowel diseases, Crohn's disease and ulcerative colitis, are chronic relapsing diseases affecting millions worldwide and disrupting their daily lives. Since polyphenols, the secondary metabolites of plants, have an antioxidant and anti-inflammatory effect, the aim of this work was to evaluate the quality of dietary supplements for the treatment of inflammatory bowel diseases with regard to the content of polyphenols and to determine their antioxidant activity. The content of total polyphenols in the analyzed samples was determined by the spectrophotometric method using the Folin-Ciocalteu reagent, while the antioxidant activity was assessed by the DPPH test. The content of polyphenols was significantly higher in a multi-botanical preparation of three plant species: turmeric, Indian frankincense and black pepper. Furthermore, the content of these biologically active substances in turmeric monopreparations was higher than in other analyzed monopreparations. The obtained results point to the exceptional quality of dietary supplements for the treatment of inflammatory bowel diseases, and the studied samples can be considered a good source of polyphenolic components, while the antioxidant activity of the tested dietary supplements is in accordance with the content of these biologically active components.

• Keywords: polifenoli; DPPH; dodaci prehrani; upalne bolesti crijeva; kurkuma; justicija; indijski tamjanovac
• Keywords (english): polyphenols; DPPH; dietary supplements; inflammatory bowel diseases; turmeric; green chiretta; indian frankincense
• Language: croatian
• URN:NBN: urn:nbn:hr:163:686414
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-09-02
• Created on: 2022-09-05 15:09:22