Određivanje sadržaja metala u cvijetu i stabljici smilja primjenom EDXRF, TXRF i ICP-MS tehnike

master's thesis

• Title (english): Determination of metal content in immortelle flower and stem using EDXRF, TXRF AND ICP-MS techniques
• Author: Ema Štefanko
• Mentor: Jasna Jablan (mentor)
• Committee member: Jasna Jablan (predsjednik povjerenstva)
• Committee member: Jerka Dumić (član povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of analytical chemistry); Zagreb
• Defense date and country: 2022-09-01, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Smilje (Helichrysum italicum (Roth) G. Don) je mediteranska cvjetnica koja se zbog svojih ljekovitih i aromatičnih svojstava koristi za izradu farmaceutskih i kozmetičkih pripravaka. Razvoj brze i osjetljive tehnike elementne analize biljnog materijala mogao bi značajno doprinijeti kakvoći i stabilnosti pripravaka te smanjiti nepotrebno izlaganje potencijalno toksičnim teškim metalima. Konvencionalne tehnike elementne analize skupe su, dugotrajne i zahtijevaju prethodnu razgradnju složenog biljnog matriksa. TXRF je mlada analitička tehnika koja vodi brigu o načelima ,,zelene kemije“, zahtjeva male količine uzorka i reagensa, a omogućava brzu i osjetljivu analizu stoga bi se mogla primjenjivati i u rutinskim analizama. Cilj ovog rada bila je usporedba rezultata dobivenih određivanjem sadržaja elemenata (Fe, Mn, Ti, Cr, Ni, Cu, Zn, Pb, Br, Se, Rb i Sr) prisutnih u uzorcima smilja primjenjujući različite analitičke metode pripreme uzoraka (suspenzija, mikrovalna digestija i pelet) i analitičke tehnike (TXRF, EDXRF i ICP-MS). Dobiveni rezultati pokazali su kako primijenjene tehnike kao ni različite metode pripreme uzoraka ne utječu značajno na rezultate analize. Pritom se TXRF pokazala kao dobra alternativna analitička tehnika budući da omogućava identifikaciju i kvantifikaciju vrlo niskih koncentracija elemenata prisutnih u uzorku bez prethodne složene kemijske obrade.

Abstract (english)

Immortelle (Helichrysum italicum (Roth) G. Don) is a Mediterranean flowering plant that, due to its medicinal and aromatic properties, is used to make pharmaceutical and cosmetic preparations. The development of a fast and sensitive technique for elemental analysis of plant material could contribute significantly to the quality and stability of preparations and reduce unnecessary exposure to potentially toxic heavy metals. Conventional elemental analysis techniques are expensive, time-consuming and require prior decomposition of the complex plant matrix. TXRF is a young analytical technique that incorporates the principles of "green chemistry", requires small amounts of samples and reagents, and provides fast and sensitive analysis, so that it could be used in routine analyses. The aim of this work was to compare the results obtained by determining the content of elements (Fe, Mn, Ti, Cr, Ni, Cu, Zn, Pb, Br, Se, Rb and Sr) present in immortelle samples using different analytical methods of sample preparation (suspension, microwave digestion and pellet) and analytical techniques (TXRF, EDXRF and ICP-MS). The obtained results showed that the techniques used as well as the different sample preparation methods did not significantly affect the analytical results. Thus, TXRF proved to be a good alternative analytical technique, since it allows the identification and quantification of very low concentrations of the elements present in the sample without prior complex chemical processing.

• Keywords: smilje; Helichrysum italicum; TXRF; EDXRF; ICP-MS
• Keywords (english): immortelle; Helichrysum italicum; TXRF; EDXRF; ICP-MS
• Language: croatian
• URN:NBN: urn:nbn:hr:163:951489
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-09-01
• Created on: 2022-09-13 09:17:10