HPLC analiza fenolnih kiselina i flavonoidnih aglikona u biljnim vrstama: Betula pendula, Salvia officinalis i Vaccinium myrtillus

master's thesis

• Title (english): HPLC analysis of phenolic acids and flavonoid aglycones in Betula pendula, Salvia officinalis and Vaccinium myrtillus
• Author: Nina Šoštarić
• Mentor: Marijana Zovko Končić (mentor)
• Committee member: Marijana Zovko Končić (predsjednik povjerenstva)
• Committee member: Suzana Inić (član povjerenstva)
• Committee member: Maja Ortner Hadžiabdić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2016-04-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U ovom radu korištena je tekućinska kromatografija visoke djelotvornosti (HPLC) za kvalitativnu i kvantitativnu analizu fenolnih kiselina i flavonoidnih aglikona u tri biljne vrste: Betula pendula, Salvia officinalis i Vaccinium myrtillus koje se koriste u narodnoj medicini za liječenje ili ublažavanje tegoba šećerne bolesti. Detekcija ispitivanih fenolnih kiselina i flavonoidnih aglikona izvršena je pomoću DAD detektora. U ispitivanim biljnim vrstama pronađene su brojne fenolne kiseline i flavonoidi poput ružmarinske i klorogenske kiseline (list kadulje, list borovnice), protokatehuične kiseline (hidrolizirani ekstrakti lista breze i lista borovnice), kavene kiseline (hidrolizirani ekstrakti lista kadulje i lista borovnice), luteolina (list kadulje) i derivata kvercetina (list breze, list borovnice, list kadulje). Prisutnost navedenih fenolnih kiselina i flavonoidnih aglikona djelomično opravdava primjenu ovih biljnih vrsta u terapiji šećerne bolesti, ali pošto je većina studija provedena in vitro ili na animalnim modelima, da bi se njihova učinkovitost potvrdila potrebno je provesti detaljne kliničke studije.

Abstract (english)

In this study, HPLC was used for qualitative and quantitative analysis of phenolic acids and flavonoid aglycones in Betula pendula, Salvia officinalis and Vaccinium myrtillus, which are used in folk medicine for the treatment or alleviation of symptoms of diabetes. Detection of tested phenolic acids and flavonoids was performed with DAD-detector. Numerous phenolic acids and flavonoids were found in these plant species, such as rosmarinic and chlorogenic acid (sage leaf,
blueberry leaf), protocatechuic acid (hydrolyzed extracts of birch leaf and blueberry leaf), caffeic acid (hydrolyzed extracts of sage leaf and blueberry leaf), luteolin (sage leaf) and quercetin derivatives (birch leaf, blueberry leaf, sage leaf). The presence of said phenolic acids and flavonoid aglycones partly justifies the application of these plant species in the treatment of diabetes, but since most studies were conducted in vitro or in animal models, to confirm their effectiveness it is necessary to carry out detailed clinical studies.

• Keywords: HPLC; fenolne kiseline; flavonoidni aglikoni; Betula pendula; Salvia officinalis; Vaccinium myrtillus; dijabetes
• Keywords (english): HPLC; phenolic acids; flavonoid aglycones; Betula pendula; Salvia officinalis; Vaccinium myrtillus; diabetes
• Language: croatian
• URN:NBN: urn:nbn:hr:163:705253
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-06-07 15:23:16