Valorizacija komine rajčice kao izvora nutritivno važnih minerala

master's thesis

• Title (english): Valorization of tomato pomace as a source of nutritionally important minerals
• Author: Borna Gašparac
• Mentor: Dubravka Vitali Čepo (mentor)
• Mentor: Jasna Jablan (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Jasna Jablan (član povjerenstva)
• Committee member: Lovorka Vujić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of analytical chemistry); Zagreb
• Defense date and country: 2023-07-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Godišnje u svijetu zaostane oko 8 milijuna tona otpada od prerade rajčice što ima negativne učinke na
okoliš te, s obzirom na činjenicu da je bogata esencijalnim nutrijentima i biološki vrijednim
fitokemikalijama predstavlja, veliki ekonomski gubitak.
Osnovni ciljevi ovog rada su istražiti primjenjivost rendgenske fluorescencijske spektrometrije s totalnom
refleksijom (TXRF) i zelenih tehnika pripreme uzorka u istraživanju mineralnog sadržaja različitih frakcija
komine rajčice sušenih u različitim uvjetima (sušenje u termostatu na 70 C i sušenje smrzavanjem).
Rezultati optimizacije metode ukazuju da kombinacija 1%-tnog Triton-a X-100®
uz izostavljanje koraka
soniciranja i volumen uzorka od 8 µL daje najtočnije rezultate.
Rezultati istraživanja su pokazali da se TXRF može koristiti u određivanju mineralnog sadržaja analiziranih
uzoraka te da priprema uzorka zelenim procesom suspendiranja daje jednako točne rezultate kao i
konvencionalne tehnike koje karakterizira puno veći ekološki otisak. Mineralni sadržaj komine rajčice u
skladu je s dostupnim literaturnim podacima, a način sušenja uzorka ne utječe značajno na mineralni sadržaj
analiziranih uzoraka. Rezultati ovog istraživanja doprinijeti će trenutnim saznanjima o prednostima i
nedostacima navedene analitičke tehnike te daljnjoj valorizaciji komine rajčice kao sekundarne sirovine
bogate nutritivno važnim mineralima.

Abstract (english)

Annually, approximately 8 million tons of tomato processing waste is generated worldwide, which has a
negative environmental impact. Considering how rich in essential nutrients and biologically valuable
phytochemicals this waste is, this represents a significant economic loss.
The main objectives of this study are to investigate the applicability of total reflection X-ray fluorescence
spectrometry (TXRF) and green sample preparation techniques in exploring the mineral composition of
different fractions of tomato pomace dried under different conditions (thermostatic drying at 70°C and
freeze-drying). The results of the method optimization indicate that the combination of 1% Triton
X-100® with omission of sonication and sample volume of 8 µL gives most accurate results.
The results of this research have demonstrated that TXRF can be utilized for determining the mineral
composition of the analyzed samples. Furthermore, sample preparation using green suspension methods
yields equally accurate results as conventional techniques, characterized by a much larger ecological
footprint. The mineral composition of tomato pomace is in accordance with available literature data, and the
drying method does not significantly affect the mineral composition of the analyzed samples. The findings
of this research could contribute to current knowledge regarding the advantages and limitations of the
aforementioned analytical technique and further valorization of tomato pomace as a secondary resource rich
in nutritionally important minerals.

• Keywords: komina rajčice; liofilizacija; mikrovalna digestija; ICP-MS; TXRF; zelena kemija; minerali
• Keywords (english): tomato pomace; lyophiliszation; microwave digestion; ICP-MS; TXRF; green chemistry; minerals
• Language: croatian
• URN:NBN: urn:nbn:hr:163:588915
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-11-21 14:39:44