Interakcija albumina s nanočesticama srebra različite površinske funkcionalizacije

master's thesis

• Title (english): Interaction of albumin and silver nanoparticles with different surface functionalization
• Author: Tea Crnković
• Mentor: Valerije Vrček (mentor)
• Committee member: Valerije Vrček (predsjednik povjerenstva)
• Committee member: Sandra Šupraha Goreta (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of organic chemistry); Zagreb
• Defense date and country: 2016-06-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

U današnjim predkliničkim testiranjima farmaceutskih molekula, među kojima se nalaze i
nanočestice za biomedicinsku primjenu, evaluacija vezanja proteina plazme je najvažniji element
kod određivanja efikasnosti, sigurnosti i raspolaganja s tim molekulama. Zbog toga je u ovom
istraživanju primijenjen modelni sustav koji se sastojao od goveđeg serumskog albumina (BSA) i
5 vrsta srebrnih nanočestica (AgNP) s različitim površinskim omotačima. Karakterizacija i
stabilnost AgNP te njihova interakcija s BSA praćeni su metodama dinamičkog i
elektroforetskog raspršenja svjetlosti, transmisijskom elektronskom mikroskopijom, UV-Vis
spektrofotometrijom te spektrofluorimetrijom. Otkriveno je da BSA dovodi do koloidne
stabilizacije AgNP u fosfatnom puferu neovisno o njihovom kemijskom sastavu, strukturi
površine i površinskom naboju. Također su dobivene različite konstante vezanja AgNP i BSA
ovisno o površinskoj funkcionalizaciji AgNP i korištenom mediju. Time je potvrđena hipoteza
da biomolekule poput BSA mogu pružiti stabilizaciju nanočestica u biološkom okruženju.

Abstract (english)

In today's preclinical testings of pharmaceutical molecules, among which are also present
nanoparticlas for biomedical applications, evaluation of plasma protein binding is one of the
most important elements in efficency, safety and distribution determination. Therefore, in this
thesis a model system of bovine serum albumin (BSA) and 5 types of silver nanoparticles
(AgNP) with different surface coatings was used. Characterization and stability of AgNP along
with their interaction with BSA were determined by using dynamic and electrophoretic light
scattering methods, transmission electron microscopy, UV-Vis spectrophotometry, and
spectrofluorimetry. It was discovered that BSA provided colloidal stability for AgNP in
phosphate buffer regardless of their chemical composition, surface structure and surface charge.
Also, different binding constants of BSA and AgNP were obtained depending on AgNP surface
functionalisation and used medium. Thereby, a hypothesis which states that biomolecules like
BSA can enhance stabilization of nanoparticles in biological environment was confirmed.

• Keywords: srebro; nanočestice; albumin; biološki medij; stabilnost
• Keywords (english): silver; nanoparticles; albumin; biological medium; stability
• Language: croatian
• URN:NBN: urn:nbn:hr:163:063082
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-12-02 14:18:43