Razvoj oftalmičkih nanoemulzija ricinusovog ulja stabiliziranih neionskim surfaktantima

master's thesis

• Title (english): Development of ophthalmic castor oil nanoemulsions stabilized by non-ionic surfactants
• Author: Matea Krpan
• Mentor: Jasmina Lovrić (mentor)
• Committee member: Jasmina Lovrić (predsjednik povjerenstva)
• Committee member: Anita Hafner (član povjerenstva)
• Committee member: Dubravka Vitali Čepo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2017-06-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Najveći nedostatak konvencionalnih oftalmičkih farmaceutskih oblika njihova je slaba bioraspoloživost, a koja je posljedica barijera prednjeg dijela oka. Nanoemulzije su sustavi s kapljicama nanometarskih dimenzija, a koje imaju izraženi potencijal za uspješnije savladavanje spomenutih barijera.
Cilj ovog ispitivanja bio je da se metodom mikrofluidizacije izrade U/V nanoemulzije ricinusova ulja stabilizirane različitim vrstama neionskih surfaktanata. U tu svrhu određivani su prosječna veličina kapljica, indeks polidisperznosti i zeta-potencijal nanaoemulzija. Rezultati su pokazali da se povećanjem količine surfaktanta veličina kapljica unutarnje faze nanoemulzije smanjuje. Količine surfaktanata od 0,5% i 1% ukupne mase emulzije pokazale su se nedovoljnima za dobivanje sustava zadovoljavajućih svojstava. Porastom masenog udjela surfaktanta (2,5% i više) primijećen je plato kod koncentracije od oko 4% i približne veličine kapljica 250 – 200 nm. Daljnjim povećanjem masenog udjela surfaktanta u nanoemulziji ne bi se postigle značajno manje veličine kapljica. Najmanju prosječnu veličinu čestica pokazale su nanoemulzije kod kojih je kao surfaktant korišten Cremophor EL. Najmanje vrijednosti indeksa polidisperznosti pokazale su nanoemulzije kod kojih je kao surfaktant korišten Pluronic F68. Ispitivanje zeta-potencijala svih formulacija ukazuje na postojanje slabog negativnog naboja na površini kapljica unutarnje faze koji potječe od slobodnih masnih kiselina porijeklom iz ricinusova ulja. Sve nanoemulzije pokazale su stabilnost tijekom 10 dana, ali nanoemulzijama s Polisorbatima 80 i 60 te s Cremophorom EL kod ponovljenog mjerenja porasla je polidisperznost čestica unutarnje faze što bi moglo učiniti nanoemulzije nestabilnima kroz duže vrijeme.

Abstract (english)

The biggest disadvantage of conventional ophtalmic forumulations lies in their low bioavailability which is the restricted due to the ocular barriers in the anterior parts of the eye. Nanoemulsions are a drug delivery system characterized by the droplets with a droplet size in the nanometer range and with the great potential to overcome these barriers. The goal of this research was to prepare O/W type nanoemulsions containing castor oil and four types of non-ionic surfactants using the microfluidization method. The average droplet size, polydispersity index as well as the zeta-potential of nanoemulsions were determined. The results obtained suggested the increase in surfactant quantity causes a decrease in internal phase droplet size. Surfactant quantities of 0,5% and 1% (of total emulsion mass) showed to be insufficient for preparing nanoemulsions of adequate properties. With growth of the surfactant mass percentage, a plateau was noticed at a concentration of around 4% and droplet sizes of approximately 250 – 200 nm. Further growth of surfactant mass percentage would not result in ensuring significantly smaller droplet sizes. Nanoemulsions containing Cremophor EL as a surfactant proved to have droplets of the smallest average size. The smallest polydispersity index (PDI) values were observed in nanoemulsions containing Pluronic F68 as a surfactant. Zeta-potential measurements of all formulations indicate the existence of low negative charge at the surface of internal phase droplets. This charge originates from free fatty acids that come from castor oil. All of the nanoemulsions were shown to be stable for 10 days, but there was an increase in PDI values noticed after remeasuring the properties of nanoemusions containing Polysorbate 80, Polysorbate 60 and Cremophor EL. Polydispersity of these formulations could result in nanoemulsion destabilisation over a longer period of time.

• Keywords: nanoemulzije; metoda mikrofluidizacije; oftalmička primjena; ricinusovo ulje; Polisorbat 80; Polisorbat 60; Pluronic F68; Cremophor EL
• Keywords (english): nanoemulsions; microfluidization method; ophthalmic application; castor oil; Polysorbate 80; Polysorbate 60; Pluronic F68; Cremophor EL
• Language: croatian
• URN:NBN: urn:nbn:hr:163:234576
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-09-15 07:53:04