Analiza kiralnih lijekova kapilarnom elektroforezom

Professional paper - Review paper

• Author: Miranda Sertić
• Author: Biljana Nigović
• Author: Mario Jug
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis)
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (croatian)

The importance of enantiomer purity control was perceived in 1960s after the Thalidomide
disaster. Enantiomers can show different pharmacological efficiency, bioavailability and therapeutic indications. Very often only one enantiomer is active, while the other can have no activity whatsoever, or can even be toxic.
Therefore, methods that enable separation and purity evaluation of enantiomers are
very important. Most often used techniques are polarimetry, circular dichroism, roentgen
diffraction and chiral chromatography. Separation techniques frequently used are
high performance liquid chromatography, gas chromatography and capillary electrophoresis.
Capillary electrophoresis is a very fast and cheap technique. The most often used
chiral separators in capillary electrophoresis are ciclodextrins. They are cyclic oligosaharyds,
composed of a-D-glucopyranoside units bound together in a ring. Topologically
cyclodextrins are represented as toroids with the larger and the smaller openings of the
toroid exposing to the solvent secondary and primary hydroxyl groups respectively. The
exterior of the toroid is hydrophilic making it water soluble, while the interior is relatively
hydrophobic. This enables the cyclodextrin to form inclusion complexes with drug molecules.
One of the enantiomers forms stronger interactions with cyclodextrin then the other cyclodextrin, which ensures enantiomer separation. Enantioselective capillary electrophoretic method using cyclodextrins are used
in pharmaceutical analysis, cosmetic and food industry, in forensic science and in environmental
protection. In pharmaceutical analysis they are used for determination of
stereochemical purity, especially when it is necessary to quantify one of the enanitomers
in the presence of large quantity of other stereoisomers.

• Keywords (croatian): kiralni lijekovi; kapilarna elektroforeza
• Language: croatian
• Publication type: Professional paper - Review paper
• Publication status: Published
• Peer review: Peer review - domestic
• Publication version: Published version
• Journal title: Farmaceutski glasnik
• Numbering: vol. 66, no. 9, pp. 467-476
• p-ISSN: 0014-8202
• URN:NBN: urn:nbn:hr:163:396520
• Publication: 2010
• Type of resource: Text
• Access conditions: Open access
• Created on: 2017-11-28 13:44:05