Učinak rekreacijskog ronjenja na plazmatsku koncentraciju galektina-3

master's thesis

• Title (english): Effect of recreational SCUBA diving on galectin-3 plasma concentration
• Author: Irena Dobrović
• Mentor: Jerka Dumić (mentor)
• Committee member: Jerka Dumić (predsjednik povjerenstva)
• Committee member: Nada Vrkić (član povjerenstva)
• Committee member: Sandra Šupraha Goreta (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology); Zagreb
• Defense date and country: 2018-06-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Galektin-3 (Gal-3) je -galaktozidni lektin kojeg eksprimiraju brojne stanice u organizmu, a posreduje u brojnim fiziološkim (apoptoza, proliferacija i diferencijacija stanica, stanična signalizacija) i patofiziološkim (upala, fibroza, karcinogeneza) procesima u organizmu. Intenzivno se istražuje kao biomarker za kardiovaskularne bolesti, fibrozu i različite tumore, koristi se u kliničke svrhe za procjenu rizika od zatajenja srca te u dijagnostici tumora štitnjače. Neke studije pokazale su da fizička aktivnost uzrokuje porast koncentracije galektina-3 u krvi, a cilj ovog istraživanja je bio otkriti na koji način rekreacijsko ronjenje s komprimiranim plinskim smjesama utječe na njegovu plazmatsku koncetraciju. U istraživanju je sudjelovalo 10 ronilaca, muškaraca životne dobi od 30 do 52 godine kojima je to bio prvi zaron nakon zimskog razdoblja neronjenja. Uzorci krvi uzeti su venepunkcijom na K2EDTA neposredno prije i nakon 30-minutnog ronjenja na 30 metara dubine te 3 i 6 sati nakon zarona. U uzorcima plazme mjerene su koncentracije Gal-3, biljega oštećenja srčanog (NT-proBNP i hs-TnI) i skeletnih (mioglobina) mišića te sistemske upale (hs-CRP). Podaci su statistički obrađeni Friedman ANOVA testom u program MedCalc. Za sve parametre osim hsCRP-a utvrđen je statistički značajan porast (p < 0.05) u plazmatskoj koncentraciji između prvog i drugog mjerenja, tj. neposredno nakon zarona. Utvrđeno je i da je koncentracija Gal-3 klinički značajno viša 3 sata nakon zarona u odnosu na vrijednost prije zarona; a koncentracije NT-proBNP i hsTnI su osim 3 sata nakon, klinički značajno više i 6 sati poslije zarona. Ovim istraživanjem prvi put je pokazano da ronjenje uzrokuje oslobađanje Gal-3 u cirkulaciju. Izvor može biti srčani mišić s obzirom na paralelno otpuštanje NT-proBNP-a i hs-TnI koji su specifični za kardiomiocite, ali i skeletni mišić s obzirom na porast koncentracije mioglobina.

Abstract (english)

Galectin-3 (Gal-3) is β-galactoside lectin expressed in wide range of cells which mediates different physiological (apoptosis, proliferation and differentiation, signalling) and pathophysiological (inflammation, fibrosis, cancerogenesis) events. Its role as biomarker in cardiovascular diseases, organ fibrosis and different tumors has been intensively studied. It is being used as a biomarker for risk assessment of heart failure and as a tool in management of thyroid nodules. Some studies showed that Gal-3 increases after physical acitivity, so the aim of this study was to investigate potential impact of recreational SCUBA diving on Gal-3 plasma levels. Ten healthy men, aged 30-52 years, performed a 30-minute SCUBA dive at 30 m depth which was their first dive after a winter non-dive period. Venous blood samples taken on anticoagulant K2EDTA were obtained from the subjects before and immediately after the dive, 3 h and 6 h after diving. In plasma samples, taken in vacuum tubes, Gal-3, biomarkers of cardiac injuries (NT-proBNP, hsTnI), skeletal muscle damage (myoglobin) and systemic inflammation (hsCRP) were measured. The obtained data were statistically analyzed in MedCalc using Friedman ANOVA test. A significant increase (p < 0.05) in plasma concentration was found with respect to baseline values immediately after diving for every parameter except for hsCRP. It was also found that Gal-3 levels were higher 3 h after diving with respect to baseline levels in clinically important settings, as for NT-proBNP and hsTnI which also showed clinically important higher values 6 h post-dive. This research showed for the first time that SCUBA diving causes Gal-3 release into the blood. The results suggest that Gal-3 might originate from heart since there is an increase in NT-proBNP and hsTnI (which are specific for cardiomyocytes); but from skeletal muscle since myoglobin concentration raise as well.

• Keywords: galektin-3; SCUBA ronjenje; srčano/mišićno oštećenje
• Keywords (english): galectin-3; SCUBA diving; cardiac/muscle damage
• Language: croatian
• URN:NBN: urn:nbn:hr:163:296098
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-11-28 16:25:55