Kvalitativna i kvantitativna analiza fenolnih kiselina vrste Laurus nobilis L. (Lauraceae) s područja Dalmacije

master's thesis

• Title (english): Qualitative and quantitative analysis of phenolic acids of Laurus nobilis L. (Lauraceae) from the Dalmatian region
• Author: Božica Svalina
• Mentor: Renata Jurišić Grubešić (mentor)
• Committee member: Renata Jurišić Grubešić (predsjednik povjerenstva)
• Committee member: Živka Juričić (član povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2018-04-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy

Abstract

U okviru ovoga diplomskog rada provedena je kvalitativna i kvantitativna analiza fenolnih kiselina vrste Laurus nobilis L. (Lauraceae) s područja Dalmacije/južnog Jadrana (populacije: Brač, Konavle, Korčula, Lastovo, Mljet, Peljašac i Šipan). Kvalitativna analiza fenolnih kiselina (ferulične, kavene, klorogenske i ružmarinske) primjenom tankoslojne kromatografije nije sa sigurnošću potvrdila njihovu prisutnost. Kvantitativna analiza fenolnih kiselina provedena je spektrofotometrijski te je sadržaj mjeren kao ružmarinska kiselina (505 nm) i klorogenska kiselina (525 nm). Maseni udio analiziranih fenolnih kiselina lovora izraženih kao ružmarinska kiselina kretao se u rasponu od 1,31% (Brač) do 3,04% (Lastovo), odnosno, izraženih kao klorogenska kiselina u rasponu od 2,52% (Brač) do 5,59% (Lastovo). Provedena kvalitativna i kvantitativna analiza predstavlja prilog znanstvenom istraživanju vrste Laurus nobilis L. i upotpunjuje dosadašnje spoznaje o fitoterapijskom potencijalu lovora, posebice u odnosu na sadržaj bioaktivnih fenolnih tvari.

Abstract (english)

In this work, qualitative and quantitative analysis of phenolic acids of Laurus nobilis L. (Lauraceae) from Dalmatian region (populations: Brač, Konavle, Korčula, Lastovo, Mljet, Pelješac, and Šipan) was carried out. Qualitative analysis of phenolic acids (ferulic, caffeic, chlorogenic and rosmarinic acid) using thin layer chromatography did not confirmed their presence with certainty. Quantitative analysis of phenolic acids was carried out using the spectrophotometric method. Phenolic acids were analyzed on 505 nm (expressed as rosmarinic acid) and 525 nm (expressed as chlorogenic acid). The contents of analyzed compounds from laurel leaves varied from 1.31% (Brač) to 3.04% (Lastovo) when expressed as rosmarinic acid, and from 2.52% (Brač) to 5.59% (Lastovo) when expressed as chlorogenic acid. Conducted qualitative and quantitative analysis is a contribution to the scientific study of Laurus nobilis L. and completes the existing knowledge about phytotherapeutic potential of laurel, especially in relation to the content of bioactive phenolic substances.

• Keywords: Laurus nobilis L.; fenolne kiseline; kvalitativna analiza; kvantitativna analiza; tankoslojna kromatografija; UV-Vis spektrofotometrija
• Keywords (english): Laurus nobilis L.; phenolic acids; qualitative analysis; quantitative analysis; thin layer chromatography; UV-Vis spectrophotometry
• Language: croatian
• URN:NBN: urn:nbn:hr:163:628545
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-12-12 15:18:18