Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study

Znanstveni rad - Izvorni znanstveni rad

• Autor: NATAŠA ZORIĆ
• Autor: NEVENKA KOPJAR
• Autor: KLARA KRALJIĆ
• Autor: NADA ORŠOLIĆ
• Autor: SINIŠA TOMIĆ
• Autor: IVAN KOSALEC
• Autorova ustanova: Sveučilište u Zagrebu; Farmaceutsko-biokemijski fakultet; (Zavod za mikrobiologiju)
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Farmacija; Farmacija

Sažetak (engleski)

Olive leaf extract is characterized by a high content of polyphenols (oleuropein, hydroxytyrosol and their derivatives), which is associated with its therapeutic properties. The objective of the present research was to evaluate the antifungal activity of olive leaf aqueous extract against Candida albicans ATCC 10231 and C. dubliniensis CBS 7987 strains. Minimum inhibitory concentration (MIC) of the extract was determined by several in vitro assays. The extract showed a concentration dependent effects on the viability of C. albicans with MIC value of 46.875 mg mL–1 and C. dubliniensis with MIC value 62.5 mg mL–1.
Most sensitive methods for testing the antifungal effect of the extract were the trypan blue exclusion method and fluorescent dye exclusion method while MIC could not be determined by the method according to the EUCAST recommendation suggesting that herbal preparations contain compounds that may interfere with this susceptibility testing. The fluorescent dye exclusion method was also used for the assessment of morphological changes in the nuclei of the treated cells. According to the obtained results, olive leaf extract is less effective against the tested strains than hydroxytyrosol, an olive plant constituent tested in our previous study.

• Ključne riječi (engleski): antifungal activity; Candida albicans; Candida dubliniensis; olive leaf extract
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Acta Pharmaceutica
• Brojčani podaci: vol. 66, br. 3, str. 411-431
• p-ISSN: 1330-0075
• e-ISSN: 1846-9558
• DOI: https://doi.org/10.1515/acph-2016-0033
• URN:NBN: urn:nbn:hr:163:681186
• Datum objave publikacije: 2016-09-30
• URL dokumenta: http://hrcak.srce.hr/156871
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2017-06-13 15:48:00