Spectrophotometric determination of malondialdehyde in urine suitable for epidemiological studies

Znanstveni rad - Izvorni znanstveni rad

• Autor: Tin Weitner
• Autor: Suzana Inić
• Autor: Jasna Jablan
• Autor: Mario Gabričević
• Autor: Ana-Marija Domijan https://orcid.org/0000-0001-5645-9732
• Autorova ustanova: Sveučilište u Zagrebu; Farmaceutsko-biokemijski fakultet; (Zavod za analitičku kemiju)
• Autorova ustanova: Sveučilište u Zagrebu; Farmaceutsko-biokemijski fakultet; (Zavod za opću i anorgansku kemiju)
• Znanstveno / umjetničko područje, polje i grana: PRIRODNE ZNANOSTI; Kemija; Analitička kemija

Sažetak (engleski)

A reliable method for spectrophotometric determination of urinary malondialdehyde (MDA), according to the thiobarbituric acid (TBA) assay, is described. To account for matrix interference and differences in individual urine composition, standard addition procedure was applied. The method is adequately selective (LoQ = 0.09 μM in the presence of 0.1 M creatinine and 0.5 M urea) and reliable (within-day and between-day variability of less than 5 %). The mean level of urinary MDA was 1.52 ± 0.73 µM that is in good agreement with spectrofluorometric determination (1.20 ± 0.56 μM; p = 0.085) as well as with previous studies that used HPLC. Furthermore, it is demonstrated that MDA is stabile in urine at room temperature for 24 h and when stored at –20 °C for 6 months. The described method enables simple, rapid and cost-effective determination of urinary MDA as a relevant and non-invasive marker of “whole-body” oxidative stress.
<br><a rel="license" href="http://creativecommons.org/licenses/by/4.0/"><img alt="Creative Commons License" style="border-width:0" src="https://i.creativecommons.org/l/by/4.0/80x15.png" /></a> This work is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International License</a>.

• Ključne riječi (engleski): malondialdehyde; thiobarbituric acid assay; urine; interferences; stability
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran - međunarodna recenzija
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Croatica Chemica Acta
• Brojčani podaci: vol. 89, br. 1, str. 133-139
• p-ISSN: 0011-1643
• e-ISSN: 1334-417X
• DOI: https://doi.org/10.5562/cca2902
• URN:NBN: urn:nbn:hr:163:584413
• Datum objave publikacije: 2016
• URL dokumenta: https://hrcak.srce.hr/160091
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2020-04-14 17:05:26