Liposomi s kalceinom: odabir optimalne metode priprave

Znanstveni rad - Izvorni znanstveni rad

• Autor: Nina Mandić
• Autor: Željka Vanić
• Autor: Jelena Filipović-Grčić
• Autorova ustanova: Sveučilište u Zagrebu; Farmaceutsko-biokemijski fakultet; (Zavod za farmaceutsku tehnologiju)
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Farmacija; Farmacija

Sažetak (engleski)

The aim of our study was to develop a liposomal drug
carrier system able to provide appropriate trapping efficiency of hydrophilic
substance. Since majority of hydrophilic drugs are of low molecular weight,
calcein was chosen as a model compound. To optimize the preparation of
liposomes with regard to size and entrapment efficiency, liposomes containing
calcein were prepared by different methods: conventional and modified
film methods as well as proliposome method. Additionally, a dehydration-rehydration
procedure was applied on the liposomes prepared by the both
film methods. Ali preparations were extruded through polycarbonate membrane
filters to achieve liposomes with homogenous size distribution. Regardless
of the preparation method, all extruded liposomes were of mean diameter
between 240 and 280 nm. However, encapsulation of calcein into liposomes
differed far various preparation methods. Liposomes prepared by the
modified film method could entrap more of the hydrophilic marker than
those prepared by the conventional hydration method. Although the dehydration-
rehydration procedure could slightly enhance the trapping of calcein
into liposomes prepared by conventional film method, the extremely high
encapsulation of calcein was achieved into proliposomes (> 40 %). Therefore,
the proliposome method would be the right choice of preparation procedure
far entrapment of hydrophilic substances.

• Ključne riječi (hrvatski): liposomi; kalcein
• Jezik: hrvatski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran - domaća recenzija
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Farmaceutski glasnik
• Brojčani podaci: vol. 68, br. 11, str. 697-708
• p-ISSN: 0014-8202
• URN:NBN: urn:nbn:hr:163:930783
• Datum objave publikacije: 2012
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2020-09-29 11:58:07